AUTOF MS Reagent

Introduction

In the clinical microbiology laboratories, microbial identification is conventionally done by phenotypic and biochemical analysis taking a few hours to several days depending on microbial species. MALDI-TOF MS technology makes generation of unique mass spectral fingerprints of microorganisms possible, which are mostly a snapshot of ribosomal proteins ideal for an accurate microbial identification at the species level.[1]MALDI-TOF MS can rapidly and accurately identify a wide range of microorganisms at a reasonable cost using only a portion or the entire colony and a drop of matrix solution.[2,3]The ability of MALDI-TOF MS to directly identify bacteria in positive blood cultures is also im-portant for the effective management of bloodstream infections.[4,5]

Specification

*MSA04, MSA05 will release in 2022.

Products Description

The CHCA Matrix for AUTOF MS is used in the identification of bacteria and fungi (except for filamentous fungi) along with MALDI TOF MS. The CHCA Matrix can adsorb laser energy and transfer the energy to the microbial protein to create ions. Then the ions can be detected by the detector of MALDI TOF MS thus identificating the microbial species by the machine.

Calibrator for AUTOF MS is a reagent used for calibration of AUTOF MS. The Calibrator contains ribonuclease, myoglobin and protein extracted from E. coli, which shows typical peptide and protein peaks in MALDI TOF MS.

The Sample Pretreatment Reagent is used for pretreatment of bacteria , fungi and positive blood culture samples in the identification along with MALDI TOF MS. Lysate 1 and Lysate 2 are used to destroy the cell wall of the sample and extract the ribosomal protein. 1 µl of the matrix solution is added to the spot with the extracted protein to generate the crystal.

The AUTOF MS Pretreatment Kit (Blood Culture Samples) is a reagent used for pretreatment of microorganism in culture medium before identification. The reagent breaks the blood cells from the positive blood culture to separate and enrich microorganisms, as to acquire pathogenic bacteria proteins for analysis by AUTOF MS.

The AUTOF MS Pretreatment Kit (Filamentous Fungi) is a reagent used for pretreatment of filamentous fungi in identification along with MALDI TOF MS. The reagent breaks down the cell walls of filamentous fungi to release proteins for analysis by AUTOF MS.

Sample Target Plate of Automated Mass Spectrometry Microbial Identification System (hereinafter "Sample target plate") is composed of target slide and target slide holder. Sample target plate is intended for carrying the sample during the sample pretreatment and identification process of the Automated Mass Spectrometry Microbial Identification System.

References

1. A. Croxatto, G. Prod'hom, and G. Greub, "Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology,"FEMS Microbi-ology Reviews, vol. 36, no. 2, pp. 380-407, 2012.

2. M.A. Claydon, S.N. Davey, V. Edwards-Jines, and D. B. Gordon, "The rapid identification of intact microorganisms using mass spectrometry,"Nature Biotechnology, vol. 14, no. 11, pp. 1584-1586, 1996.

3. R. D. Holland, J. G. Wilkes, F. Rafii et al., "Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry,"Rapid Communications in Mass Spectrometry, vol. 10, no. 10, pp. 1227-1232, 1996.

4. M. Drancourt, "Detection of microorganisms in blood specimens using matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a review,"Clinical Microbiology and Infection, vol. 16, no. 11, pp. 1620-1625, 2010.

5. Y. Hoyos-Mallecot, C. Riazzo, C. Miranda-Casas, M. Rojo Martín, J. Gutiérrez-Fernández, and J. Navarro-Marí, "Rapid detection and identi-fication of strains carrying carbapenemasses directly from positive blood culture using MALDI-TOF MS,"Journal of Microbiological Method, vol. 105, pp. 98-101, 2014.

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MALDI-TOF MS System

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