Table of contents:
What is Chemiluminescence Immunoassay?
Chemiluminescence Immunoassay (CLIA) is a laboratory technique used to detect and quantify specific substances such as proteins, hormones, and antibodies in biological samples. This method utilizes the emission of light (chemiluminescence) during a chemical reaction, which is then measured to determine the concentration of the analyte in the sample. CLIA is widely used in clinical diagnostics due to its high sensitivity and specificity, making it ideal for detecting low levels of biomarkers in blood or other bodily fluids.
Why CLIA is Important in Medical Diagnostics?
CLIA provides several advantages in medical diagnostics:
- High Sensitivity and Specificity: CLIA offers superior accuracy and reliability, even for detecting very small amounts of target substances.
- Broad Application: This technique is used for various applications including hormone testing, infectious disease detection, and cancer biomarker assays.
- Quick Results: CLIA assays provide fast and efficient results, essential for rapid decision-making in clinical settings.
History of Immunoassay Development
Immunoassay technologies have evolved over decades to become the cornerstone of modern diagnostic testing. From the early use of radioactive methods to the current dominance of chemiluminescence, the development of immunoassay technologies has significantly advanced medical diagnostics. Below is a brief overview of how immunoassay technologies have progressed over the years:
1960s: Radioimmunoassay (RIA)
The 1960s marked the beginning of immunoassay development with the introduction of radioimmunoassay (RIA). This method was widely used due to its low cost and high sensitivity. However, it had significant drawbacks, such as radioactive contamination, short shelf life of reagents, and complex operation procedures. These limitations made RIA less ideal for widespread use in clinical diagnostics.
1970s: Colloidal Gold Technology
In the 1970s, colloidal gold technology was introduced, offering a much simpler and faster process. This advancement was a breakthrough for point-of-care testing due to its ease of use. However, it struggled with low sensitivity, which remained a major issue due to the inherent limitations of the technology itself.
1980s: Enzyme-Linked Immunosorbent Assay (ELISA) & Time-Resolved Fluorescence
In the 1980s, Enzyme-Linked Immunosorbent Assay (ELISA) and time-resolved fluorescence (TRF) emerged as more advanced immunoassay techniques. ELISA, which was primarily developed by domestic manufacturers like Shanghai Kehua, gained popularity due to its low cost and stable properties. However, it could not meet the increasing demand for higher sensitivity and precision, which limited its application for complex diagnostics. On the other hand, time-resolved fluorescence (TRF), as used by companies such as Shanghai Xinnuo, showed improvements in sensitivity but introduced its own challenges, including complex operations, high cost, and long reaction times. Despite these shortcomings, TRF became the foundation for further development in immunoassay technologies, particularly in the domestic market.
1990s: Emergence of Chemiluminescence
The 1990s saw the introduction of chemiluminescence immunoassay (CLIA), a revolutionary technology that quickly gained attention for its high sensitivity, fast detection times, and versatility. While the adoption of CLIA was slow in domestic markets, it gradually gained popularity, especially after the 21st century. With its rise, the market for CLIA-based systems began to expand rapidly, particularly in clinical diagnostics where precision and speed are crucial.
2000s: CLIA Becomes the Mainstream Immunoassay Technology
By the 2010s, CLIA had firmly established itself as the leading technology in immunoassay diagnostics, replacing previous methods like time-resolved fluorescence. The widespread adoption of CLIA was driven by its superior performance and the ability to handle a broader range of diagnostic tests with greater accuracy. Today, CLIA is the dominant method in the immunoassay market, enabling faster, more reliable diagnoses and contributing to better patient outcomes.
Call to Action: Autobio, as a leading manufacturer of high-performance CLIA analyzers, is at the forefront of these innovations. Our CLIA analyzers are designed to provide unparalleled precision and efficiency in diagnostic testing. Contact us today to learn more about how our products can improve your laboratory's diagnostic capabilities and enhance patient care.
Autobio, as a leading manufacturer of high-performance CLIA analyzers, is at the forefront of these innovations. Our CLIA analyzers are designed to provide unparalleled precision and efficiency in diagnostic testing. Contact us today to learn more about how our products can improve your laboratory's diagnostic capabilities and enhance patient care.
Basic Principles of Chemiluminescence (CL) Analysis
Chemiluminescence (CL) is a type of molecular luminescence spectroscopy that relies on the principle of a linear quantitative relationship between the concentration of the substance to be measured and the chemical luminescence intensity under specific conditions. This method uses instruments to detect the system's chemical luminescence intensity to determine the concentration of the target substance. Chemiluminescence is a trace analysis method that distinguishes itself from other types of luminescence analysis in that the energy source for the emitted light (radiation) comes from a chemical reaction, rather than from external light sources. In simple terms, chemiluminescence refers to the phenomenon of light emission accompanying a chemical reaction. It occurs when a chemical reaction generates enough energy to emit light, and the light emitted is used for detecting the concentration of a substance.
Types of Chemiluminescence Analysis Based on Energy Supply Reactions
Chemiluminescence analysis methods can be classified based on the characteristics of the energy-supplying reactions:
- Conventional Chemiluminescence Analysis (the energy-supplying reaction is a general chemical reaction)
- Biochemiluminescence Analysis (the energy-supplying reaction is a biochemical reaction, also known as BCL)
- Electrochemiluminescence Analysis (the energy-supplying reaction is an electrochemical reaction, abbreviated as ECL)
Autobio's Chemiluminescence Reagents: Perfecting Diagnostics
Autobio also offers a wide range of high-quality chemiluminescence reagents that are designed for use with our analyzers. These reagents are formulated to provide optimal results for various diagnostic tests, including infectious disease markers, hormone analysis, and oncology testing. The high stability and reliability of Autobio's reagents make them the ideal choice for laboratories seeking consistent performance in their diagnostic assays.
Autobio's Chemiluminescence Immunoassay (CLIA) Analyzers
At Autobio, we specialize in providing high-performance CLIA-compliant analyzers that meet the rigorous standards of clinical diagnostic laboratories. Our products are certified with ISO 9001, ISO 13485, ISO 17025, and CE IVDR certifications, ensuring compliance with international quality and regulatory standards.
Key Features of Autobio's CLIA Analyzers
- Versatile Testing: Autobio's CLIA analyzers are designed to handle a wide range of tests, including all chemiluminescence immunoassay reagent kits, allowing laboratories to conduct various diagnostic tests on a single platform.
- Cost-Effective: Our analyzers are priced competitively, providing laboratories with an affordable solution without compromising on quality or performance.
- High Throughput Capacity: Autobio's analyzers support high-throughput testing, making them ideal for busy hospital labs and diagnostic centers that require rapid processing of large volumes of samples.
- Global Reach: Autobio offers international shipping and insurance based on CPT export norms, ensuring easy access to our products worldwide.
How Autobio's CLIA Analyzers Improve Diagnostic Efficiency
Autobio's CLIA analyzers are equipped with advanced automation features that streamline laboratory workflows and reduce the risk of human error. By automating diagnostic tests, our analyzers significantly speed up testing processes, ensuring faster results and more accurate diagnoses. Whether performing routine immunoassays or complex biomarker analysis, Autobio's products enhance laboratory efficiency and contribute to better patient outcomes.
For example: A6 Series Analyzer: High Performance for Diagnostic Testing
Autobio's A6 Series Chemiluminescence Analyzer offers a range of innovative features designed to enhance diagnostic testing efficiency. Key highlights include: - Dual-Core Backup System: Ensures reliable operation with backup designs for critical components, minimizing downtime.
- High-Speed Detection: With a detection speed of 600 tests per hour per module, and up to 2400 tests per hour when linked, it supports fast, high-throughput diagnostics.
- Convenient and Safe Operation: Allows for reagent replacement without stopping the machine, supports automatic cleaning liquid preparation, and includes bio-safe designs to prevent contamination.
- Comprehensive Test Menu: Capable of performing over 100 different tests, including tumor markers, liver diseases, infections, and more, making it ideal for diverse diagnostic needs.
The A6000 analyzer is an advanced, reliable solution for modern laboratories, helping improve workflow and diagnostic accuracy.
Why Choose Autobio for Your CLIA Analyzer Needs?
Autobio offers industry-leading solutions for chemiluminescence immunoassay testing. Our analyzers are backed by global certifications and offer the perfect balance of performance, versatility, and affordability. Whether you're a hospital laboratory, a diagnostic center, or a medical distributor, Autobio's CLIA analyzers are the ideal choice for high-quality, reliable diagnostic equipment. Contact Autobio today to learn more about our CLIA-compliant analyzers and how our advanced, cost-effective solutions can improve your laboratory's diagnostic capabilities.
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